Chemically defined medium for the growth of Pasteurella tularensis.

نویسندگان

S C NAGLE

R E ANDERSON

N D GARY

چکیده

Chemically defined media for the growth of Pasteurella tularensis have been described by Tamura and Fleming (1949) and more recently by Giesken (1954), Mager et al. (1954), and Traub et al. (1955). Upon application of the latter medium (Traub et al.) for the growth of a virulent strain of P. tularensis, it was noted that the viable cell yields were markedly lower than yields previously obtained in a complex casein hydrolyzate medium similar to that reported by Mfills et al. (1949). This observation led to an investigation of the nutritional requirements of several strains of P. tularensis, for the purpose of devising a medium which would support the growth of virulent strains in yields comparable to those obtained in the complex medium.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of Live Tularemia Vaccine Prepared in a Chemically Defined Medium.

A chemically defined medium was prepared which adequately supported growth of a vaccine strain of Pasteurella tularensis. This medium differed from those previously described in: (i) concentration of components, (ii) a requirement for calcium pantothenate to obtain increased growth, and (iii) a low initial pH. Varying the concentration of individual components up to 10 times the standard amount...

متن کامل

A growth medium without blood cells for Pasteurella tularensis.

متن کامل

Growth and metabolism of live vaccine strain of Pasteurella tularensis.

The growth and metabolism of the live vaccine strain of Pasteurella tularensis in different media were investigated. Maximal growth was observed in a medium containing a sulfuric acid digest of casein as amino acid source. Amino acid metabolism produced considerable ammonia, and the rate of ammonia evolution was directly proportional to the growth rate. The most likely route for amino acid brea...

متن کامل

CmEMIcAzy DEFINED CULTURE MEDIUM FOR PASTEURELLA PESTIS

The chemically defined culture media for Pasteurella pestis which were formulated by Rao (1939, 1940a, b), Berkman (1942), Doudoroff (1943), and Herbert (1949) did not support the growth of any of the 27 strains available in this laboratory. This report describes the.development of a chemically defined medium which was evaluated in terms of the following criteria: yields of viable cells, minimu...

متن کامل